Frontiers in Microbiology (Sep 2015)

Organogel nanoemulsion containing Nisin and D-limonene and its antimicrobial activity

  • Weiya eBei,
  • Yan eZhou,
  • Xuya eXing,
  • Mohamed Red eZahi,
  • Yuan eLi,
  • Qipeng eYuan,
  • Hao eLiang

DOI
https://doi.org/10.3389/fmicb.2015.01010
Journal volume & issue
Vol. 6

Abstract

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The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity. Organogel-nanoemulsion containing with D-limonene and nisin or without nisin was prepared by a homogenization method. Factors that may affect the droplet size and stability of organogel-nanoemulsion such as pressure and surfactant to oil ratio were studied. The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low surfactant to oil ratio. Positive effects and outstanding antimicrobial activities of organogel-nanoemulsion containing with D-limonene and nisin were confirmed by MICs comparison, growth curves of bacteria, scanning electron microscopy and determination of cell constituents’ release. Furthermore, the organogel-nanoemulsion applied as food preservative in milk also shown excellent antimicrobial performance. Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.

Keywords