The Effect of Different Titers of Blood-Grouping Reagents on Proliferation and Apoptosis of Bone Marrow Derived Mesenchymal Stem Cells

Abstract

Hematopoetic Stem Cell Transplantation (HSCT) has benefical effects for the treatment of leukemia but one of the adverse effect is Graft versus Host Disease (GVHD) that causes high mortality and morbidity. Mesenchymal Stem Cells (MSCs) are one of the promising tools which are used for the treatment of steroid refractory GVHD. The success of cellular therapies depends on so many factors associated with the donor and host characteristics. We designed a study concerning that blood group reagents at different titres can affect the survival of MSCS in blood as they were injected intravenously when used for the treatment of GVHD. We concluded that proliferation rates of MSCs are decreasing in relation with the high reagent titres. Flow-cytometric analysis also revealed that high titers of reagent in culture medium could lead to the induction of apoptotic pathways in MSCs. For individualized treatment strategies, donor and host characteristics must be taken in to consideration in details and experimental studies in cell culture laboratories could be essential to gain insight into clinical trials.

Keywords

• Hematopoetic Stem Cell Transplantation (HSCT)
• Graft Versus Host Disease (GVHD)
• Mesenchymal Stem Cells (MSCs)
• apoptosis