Biosynthesized copper oxide nanoparticles (CuO NPs) enhances the anti-biofilm efficacy against K. pneumoniae and S. aureus

Abstract

In this experimental study was designed to synthesis the CuO NPs using medicinal plant of Moringa oleifera for eradicate the biofilm formation. In result, the UV-spectrum of the synthesized CuO NPs subjected to the range between 200 and 600 nm. Among these, the prominent absorbance peak at 430 nm indicated the peak was held to the copper oxide nanoparticle (CuO NPs). The particle size of biosynthesized CuO NPs varied from 90 nm to 250 nm with more than 50% distribution appearing between 130 nm and 170 nm. In addition, it was further confirmed by PSA, SEM, TEM and SAED of irregular shape and also crystalline nature. All the synthesis and characterization results were clearly supported to the result and confirmed that the synthesized nanoparticle is CuO NPs. Further, the biosynthesized CuO NPs was performed against biofilm forming K. pneumoniae, S. aureus and A. baumannii. The anti-bacterial activity result was shown with 20 mm, 14 mm and 18 mm zone of inhibition against K. pneumoniae, S. aureus and A. baumannii. The minimum biofilm inhibition concentration result was more evident, that the CuO NPs has excellent anti-biofilm activity at 1000 µg/mL concentration. Furthermore, the decreased survival rate of the CuO NPs was observed at 1000 µg /mL concentration by liquid survival degradation assay. Overall, all the results were clearly confirmed that the CuO NPs has excellent anti-biofilm ability against K. pneumoniae, S. aureus and A. baumannii.

Keywords

• Copper oxide nanoparticles
• Green synthesis
• Anti-biofilm activity
• Minimum biofilm inhibition concentration
• Biofilm survival assay