The bZIP transcription factor GmbZIP15 facilitates resistance against Sclerotinia sclerotiorum and Phytophthora sojae infection in soybean
Man Zhang,
Yanhui Liu,
Zixian Li,
Zeyuan She,
Mengnan Chai,
Mohammad Aslam,
Qing He,
Youmei Huang,
Fangqian Chen,
Huihuang Chen,
Shikui Song,
Bingrui Wang,
Hanyang Cai,
Yuan Qin
Affiliations
Man Zhang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Yanhui Liu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Zixian Li
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Zeyuan She
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China
Mengnan Chai
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Mohammad Aslam
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China
Qing He
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Youmei Huang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Fangqian Chen
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Huihuang Chen
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Shikui Song
Horticultural Plant Biology and Metabolomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Bingrui Wang
College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China; Corresponding author
Hanyang Cai
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China; Corresponding author
Yuan Qin
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, College of Plant Protection, College of Life Sciences, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China; Corresponding author
Summary: Soybean, one of the most valuable oilseed crops, is under constant pressure from pathogens. bZIP transcription factors (TFs) composing one of the largest TF families in plants have diverse functions. Biochemical and physiological analyses were performed to characterize the regulatory roles of soybean bZIP TF GmbZIP15 in response to pathogens. We found that transgenic soybean plants overexpressing GmbZIP15 has increased resistance against Sclerotinia sclerotiorum and Phytophthora sojae. Besides, GmbZIP15 regulates pathogen response by modulating the antioxidant defense system and phytohormone signaling. In addition, we performed chromatin immunoprecipitation sequencing to identify the downstream genes of GmbZIP15 in response to S. sclerotiorum and found that GmbZIP15 can activate or repress the expression of defense-related genes through direct promoter binding. Taken together, these results indicate that GmbZIP15 plays a positive role in pathogen resistance in soybean, and this activity may be dependent on phytohormone signaling.
