Two-color nanoscopy of organelles for extended times with HIDE probes

Ling Chu; Jonathan Tyson; Juliana E. Shaw; Felix Rivera-Molina; Anthony J. Koleske; Alanna Schepartz; Derek K. Toomre

doi:10.1038/s41467-020-17859-1

Nature Communications (Aug 2020)

Two-color nanoscopy of organelles for extended times with HIDE probes

Ling Chu,
Jonathan Tyson,
Juliana E. Shaw,
Felix Rivera-Molina,
Anthony J. Koleske,
Alanna Schepartz,
Derek K. Toomre

Affiliations

Ling Chu: Department of Cell Biology, Yale University School of Medicine
Jonathan Tyson: Department of Chemistry, Yale University
Juliana E. Shaw: Department of Molecular Biophysics and Biochemistry, Yale University
Felix Rivera-Molina: Department of Cell Biology, Yale University School of Medicine
Anthony J. Koleske: Department of Molecular Biophysics and Biochemistry, Yale University
Alanna Schepartz: Department of Chemistry, Yale University
Derek K. Toomre: Department of Cell Biology, Yale University School of Medicine

DOI: https://doi.org/10.1038/s41467-020-17859-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 8

Abstract

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High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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