Infection and Drug Resistance (Dec 2021)
Whole Genome Sequencing of First Janibacter indicus Isolate in China Revealed Three Unique Genomic Islands Compared with Saprophytic Strains
Abstract
Min Zhang,1,2,* Ziwen Wang,1,3,* Zhenshan Liang,4 Niya Hu2 1Department of Blood Transfusion, The Third Affiliated Hospital of Nanchang University, Nanchang City, 330006, People’s Republic of China; 2Department of Laboratory Medicine, The First Affiliated Hospital of Nanchang, Nanchang City, 330006, People’s Republic of China; 3Department of Laboratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, People’s Republic of China; 4Department of Laboratory Medicine, The Affiliated Children’s Hospital of Nanchang University, Nanchang City, 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Min Zhang; Niya Hu Email [email protected]; [email protected]: Janibacter caused bacteriemia is one of the rare infections.Methods: In the present study, we report the first isolation of Janibacter, a rare bacterial infection, from a bacteremia patient in China. Its 16S rDNA was amplified and designated as Janibacter YFY001, which belongs to J. indicus. In addition, its genome was sequenced through combined second- and third-generation genome sequencing methods.Results: Based on its genome, we identified many virulence factors, such as catalase, gelatinase, FbpABC systems, and resistant genes, among others. Interestingly, three genomic islands were found in YFY001 by comparing its genome to environmental Janibacter strains.Discussion: Our study not only provides the necessary genomic information for in-depth study of Janibacter, but also provides a novel methodology for studying future cases of rare bacterial infection.Keywords: bacteriemia, Janibacter, genomic sequencing
