Generation of Trophoblast Organoids from Chorionic Villus Sampling
Bas van Rijn,
Diane Van Opstal,
Nicole van Koetsveld,
Maarten Knapen,
Joost Gribnau,
Olivier Schäffers
Affiliations
Bas van Rijn
Department of Obstetrics and Gynaecology, Division of Obstetrics and Fetal Medicine, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands
Diane Van Opstal
Department of Clinical Genetics, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands
Nicole van Koetsveld
Department of Clinical Genetics, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands
Maarten Knapen
Department of Obstetrics and Gynaecology, Division of Obstetrics and Fetal Medicine, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands
Joost Gribnau
Department of Developmental Biology, Erasmus University Medical Center, 3015 GN Rotterdam, The Netherlands
Olivier Schäffers
Department of Obstetrics and Gynaecology, Division of Obstetrics and Fetal Medicine, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands
Studying human placental development and function presents significant challenges due to the inherent difficulties in obtaining and maintaining placental tissue throughout the course of an ongoing pregnancy. Here, we provide a detailed protocol for generating trophoblast organoids from chorionic villi obtained during ongoing pregnancy. Our method results in efficient generation of trophoblast organoids from chorionic villus sampling, does not require preselection of chorionic villi, and controls contamination of decidual gland organoids. The resulting trophoblast organoids spontaneously form syncytiotrophoblasts that start secreting hCG hormone amongst other placenta-specific factors. Our approach facilitates the generation of trophoblast organoids from a variety of genetic backgrounds, including trisomies and gene mutations, and can be aligned with prenatal diagnostic routines. The protocol requires up to 14 days and can be carried out by users with expertise in cell culture.
