Frontiers in Oncology (Feb 2020)
High Dimensional Mass Cytometry Analysis Reveals Characteristics of the Immunosuppressive Microenvironment in Diffuse Astrocytomas
- Weilun Fu,
- Weilun Fu,
- Wenjing Wang,
- Hao Li,
- Hao Li,
- Yuming Jiao,
- Yuming Jiao,
- Jiancong Weng,
- Jiancong Weng,
- Ran Huo,
- Ran Huo,
- Zihan Yan,
- Zihan Yan,
- Jie Wang,
- Jie Wang,
- Hongyuan Xu,
- Hongyuan Xu,
- Shuo Wang,
- Shuo Wang,
- Jiangfei Wang,
- Jiangfei Wang,
- Dexi Chen,
- Yong Cao,
- Yong Cao,
- Jizong Zhao,
- Jizong Zhao
Affiliations
- Weilun Fu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Weilun Fu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Wenjing Wang
- Institute of Hepatology, Capital Medical University Affiliated Beijing You'an Hospital, Beijing, China
- Hao Li
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Hao Li
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Yuming Jiao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Yuming Jiao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jiancong Weng
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jiancong Weng
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Ran Huo
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Ran Huo
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Zihan Yan
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Zihan Yan
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jie Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jie Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Hongyuan Xu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Hongyuan Xu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Shuo Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Shuo Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jiangfei Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jiangfei Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Dexi Chen
- Institute of Hepatology, Capital Medical University Affiliated Beijing You'an Hospital, Beijing, China
- Yong Cao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Yong Cao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jizong Zhao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jizong Zhao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- DOI
- https://doi.org/10.3389/fonc.2020.00078
- Journal volume & issue
-
Vol. 10
Abstract
The tumor immune microenvironment (TIME) plays a pivotal role in tumor development, progression, and prognosis. However, the characteristics of the TIME in diffuse astrocytoma (DA) are still unclear. Leveraging mass cytometry with a panel of 33 markers, we analyzed the infiltrating immune cells from 10 DA and 4 oligodendroglioma (OG) tissues and provided a single cell-resolution landscape of the intricate immune microenvironment. Our study profiled the composition of the TIME in DA and confirmed the presence of immune cells, such as glioma-associated microglia/macrophages (GAMs), CD8+ T cells, CD4+ T cells, regulatory T cells (Tregs), and natural killer cells. Increased percentages of PD-1+ CD8+ T cells, TIM-3+ CD4+ T cell subpopulations, Tregs and pro-tumor phenotype GAMs substantially contribute to the local immunosuppressive microenvironment in DA. DAs and OGs share similar compositions in terms of immune cells, while GAMs in DA exhibit more inhibitory characteristics than those in OG.
Keywords
