Efecto repelente del aceite de las semillas de Jatropha curcas L. en larvas de Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Acari: Ixodidae)

Abstract

Objective: To evaluate the repellent activity of the oil from Jatropha curcas L. seeds with different storage periods on larvae of Rhipicephalus (Boophilus) microplus. Materials and Methods: The research was conducted in the parasitology and biotechnology laboratories of the Pastures and Forages Research Station Indio Hatuey, located in the Perico municipality, Matanzas province, Cuba. The tick strain Cayo Coco was used and oils with two storage periods were evaluated: one and three years of extraction. As positive control the acaricide Deltametrina (Butox®) was used and as negative one, distilled water. For the in vitro results the methodology for the identification of repellent substances, described by Chagas and Dias (2012), was used, where the concentrations of 0,5; 1,75; 2,5; 5 and 10 mg mL-1, were used. Results: The analysis showed significant differences (p < 0,05) between the experimental treatments in the different times, with regards to the negative control (distilled water). The repellence percentages were higher than 90 % for the J. curcas oils. The repellent activity was high, and the interval of 4-8 hours was the one with the best results, with values of 93,9 and 97,3 % for the 2014 and 2017 oil, respectively, without significant differences between both. After 8 hours, the positive control decreased its repellent activity until reaching values of 64,2 % at 16 hours. The distilled water did not show repellent activity, because its values were in an interval between 3 and 7 % during the experimental stage. Conclusions: The J. curcas oil has repellent activity, independently from the storage time, with values that exceed 90 % of efficacy.

Keywords

• larvae; rhipicephalus (boophilus) microplus; animal health