Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Shih-Te Hung; Arnau Llobet Rosell; Daphne Jurriens; Marijn Siemons; Oleg Soloviev; Lukas C. Kapitein; Kristin Grußmayer; Lukas J. Neukomm; Michel Verhaegen; Carlas Smith; Carlas Smith

doi:10.3389/fnins.2022.954949

Frontiers in Neuroscience (Oct 2022)

Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Shih-Te Hung,
Arnau Llobet Rosell,
Daphne Jurriens,
Marijn Siemons,
Oleg Soloviev,
Lukas C. Kapitein,
Kristin Grußmayer,
Lukas J. Neukomm,
Michel Verhaegen,
Carlas Smith,
Carlas Smith

Affiliations

Shih-Te Hung: Delft Center for Systems and Control, Delft University of Technology, Delft, Netherlands
Arnau Llobet Rosell: Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
Daphne Jurriens: Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Marijn Siemons: Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Oleg Soloviev: Delft Center for Systems and Control, Delft University of Technology, Delft, Netherlands
Lukas C. Kapitein: Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands
Kristin Grußmayer: Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, Netherlands
Lukas J. Neukomm: Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland
Michel Verhaegen: Delft Center for Systems and Control, Delft University of Technology, Delft, Netherlands
Carlas Smith: Delft Center for Systems and Control, Delft University of Technology, Delft, Netherlands
Carlas Smith: Department of Imaging Physics, Delft University of Technology, Delft, Netherlands

DOI: https://doi.org/10.3389/fnins.2022.954949
Journal volume & issue: Vol. 16

Abstract

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Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

Published in Frontiers in Neuroscience

ISSN: 1662-4548 (Print); 1662-453X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/neuroscience

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