Nature Communications (Nov 2021)
Spike residue 403 affects binding of coronavirus spikes to human ACE2
- Fabian Zech,
- Daniel Schniertshauer,
- Christoph Jung,
- Alexandra Herrmann,
- Arne Cordsmeier,
- Qinya Xie,
- Rayhane Nchioua,
- Caterina Prelli Bozzo,
- Meta Volcic,
- Lennart Koepke,
- Janis A. Müller,
- Jana Krüger,
- Sandra Heller,
- Steffen Stenger,
- Markus Hoffmann,
- Stefan Pöhlmann,
- Alexander Kleger,
- Timo Jacob,
- Karl-Klaus Conzelmann,
- Armin Ensser,
- Konstantin M. J. Sparrer,
- Frank Kirchhoff
Affiliations
- Fabian Zech
- Institute of Molecular Virology, Ulm University Medical Center
- Daniel Schniertshauer
- Institute of Molecular Virology, Ulm University Medical Center
- Christoph Jung
- Institute of Electrochemistry, Ulm University
- Alexandra Herrmann
- Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg
- Arne Cordsmeier
- Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg
- Qinya Xie
- Institute of Molecular Virology, Ulm University Medical Center
- Rayhane Nchioua
- Institute of Molecular Virology, Ulm University Medical Center
- Caterina Prelli Bozzo
- Institute of Molecular Virology, Ulm University Medical Center
- Meta Volcic
- Institute of Molecular Virology, Ulm University Medical Center
- Lennart Koepke
- Institute of Molecular Virology, Ulm University Medical Center
- Janis A. Müller
- Institute of Molecular Virology, Ulm University Medical Center
- Jana Krüger
- Department of Internal Medicine I, Ulm University Medical Center
- Sandra Heller
- Department of Internal Medicine I, Ulm University Medical Center
- Steffen Stenger
- Institute of Medical Microbiology and Hygiene, Ulm University Medical Centre
- Markus Hoffmann
- Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research
- Stefan Pöhlmann
- Infection Biology Unit, German Primate Center—Leibniz Institute for Primate Research
- Alexander Kleger
- Department of Internal Medicine I, Ulm University Medical Center
- Timo Jacob
- Institute of Electrochemistry, Ulm University
- Karl-Klaus Conzelmann
- Max von Pettenkofer-Institute of Virology, Medical Faculty, and Gene Center, Ludwig-Maximilians-Universität München
- Armin Ensser
- Institute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg
- Konstantin M. J. Sparrer
- Institute of Molecular Virology, Ulm University Medical Center
- Frank Kirchhoff
- Institute of Molecular Virology, Ulm University Medical Center
- DOI
- https://doi.org/10.1038/s41467-021-27180-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 10
Abstract
The bat sarbecovirus RaTG13 is a close relative of SARS-CoV-2, but its spike protein doesn’t efficiently bind human ACE2. Here, the authors show that exchange of spike residue 403 between RaTG13 and SARS-CoV-2 spike proteins affects binding to human ACE2 and entry of pseudotyped viruses.
