METHOD DEVELOPMENT FOR CIPROFLOXACIN ANALYSIS IN HONEY BY LC-MS/MS

Abstract

Ciprofloxacin is a veterinary drugs authorized for use in food animal production. The analysis of residual amounts of drugs in food from animal origin is important for quality control of products for consumers. Due to its importance, a method was developed for screening and confirming ciprofloxacin residue in honey samples. Method development consisted in preparing the standard solutions, optimizing the compound and source dependent parameters, choosing the mobile phase and the appropriate HPLC column followed by processing a calibration curve and finally injecting a real sample. The honey sample was collected from Transylvania region. Its extraction consisted in acidification and dilution of the sample, extraction on SPE columns, elution with ammonium hydroxide in methanol, evaporation to dry and reconstitution with mobile phase. The extracted sample was then injected on a Phenyl-Hexyl column and detected through a LC/MS/MS system in an ESI (electrospray ionization) mode and the result was considered to be satisfying.