An initial characterisation of the Unfolded Protein Response pathway in haematopoietic canine cancer cell lines – a necessary step for the future development of new therapies in dogs with neoplasia

Abstract

New and more effective therapies for canine cancer patients are urgently required and this necessitates advanced experimental research. Dogs are good models for studies in comparative oncology; however, canine cancer cell biology research is currently limited by low availability of validated antibody reagents and techniques. This study characterises the expression of key components of the unfolded protein response (UPR) in a panel of haematopoietic canine cancer cell lines using commercially available antibodies, and validates the methods used to study this pathway.

Keywords

• canine cancer
• eukaryotic translation initiation factor 2α (eif2α)
• ccaat/enhancer binding protein homologous protein (chop)
• protein kinase rna-like endoplasmic reticulum kinase (perk)