Investigation of Red Blood Cells by Atomic Force Microscopy
Viktoria Sergunova,
Stanislav Leesment,
Aleksandr Kozlov,
Vladimir Inozemtsev,
Polina Platitsina,
Snezhanna Lyapunova,
Alexander Onufrievich,
Vyacheslav Polyakov,
Ekaterina Sherstyukova
Affiliations
Viktoria Sergunova
Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Stanislav Leesment
NT-MDT Spectrum Instruments, Proezd 4922, 4/3 Zelenograd, 124460 Moscow, Russia
Aleksandr Kozlov
Department of Medical and Biological Physics, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia
Vladimir Inozemtsev
Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Polina Platitsina
Institute of Biotechnical Systems and Technologies National Research“MIET”, Shokin Sq., Build.1, 124498 Zelenograd, Russia
Snezhanna Lyapunova
Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Alexander Onufrievich
Federal State Budgetary Institution “N.N. Burdenko Main Military Clinical Hospital” of the Ministry of Defense of the Russian Federation, Hospital Sq., Build. 3, 105094 Moscow, Russia
Vyacheslav Polyakov
NT-MDT Spectrum Instruments, Proezd 4922, 4/3 Zelenograd, 124460 Moscow, Russia
Ekaterina Sherstyukova
Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia
Currently, much research is devoted to the study of biological objects using atomic force microscopy (AFM). This method’s resolution is superior to the other non-scanning techniques. Our study aims to further emphasize some of the advantages of using AFM as a clinical screening tool. The study focused on red blood cells exposed to various physical and chemical factors, namely hemin, zinc ions, and long-term storage. AFM was used to investigate the morphological, nanostructural, cytoskeletal, and mechanical properties of red blood cells (RBCs). Based on experimental data, a set of important biomarkers determining the status of blood cells have been identified.
