Evaluation of the apoptotic effect of sinapic acid in d17 canine osteosarcoma cell line

Abstract

Sinapic acid (SA), one of the hydroxycinnamic acid derivatives, has powerful antioxidant and anti-inflammatory characteristics. Moreover, SA has also been shown to have an apoptotic effect against various cancer cells. Here, we investigated the cytotoxic and apoptotic effects of sinapic acid on D17 canine osteosarcoma cell line. We measured the properties of SA on cell viability with the XTT test and found its IC50 dose at 750 μmol at 72 h. We analyzed the effects on gene expression of apoptosis pathways by qRT-PCR. qRT-PCR results revealed significant increases in the mRNA level expressions of BAX, CASP3, CASP7, CASP8, CASP9, FAS and P53; whereas, there was a statistically insignificant downregulation in CYCS level and increase in BCL2 level. Our findings show that SA can induce apoptosis in the D17 cell line.

Keywords

• apoptosis
• canine osteosarcoma
• sinapic acid