Frontiers in Plant Science (Sep 2022)
Identification and validation of a locus for wheat maximum root length independent of parental reproductive environment
- Huangxin Chen,
- Huangxin Chen,
- Conghao Zhao,
- Conghao Zhao,
- Yaoyao Yang,
- Yaoyao Yang,
- Zhaoyong Zeng,
- Zhaoyong Zeng,
- Wei Li,
- Wei Li,
- Yanlin Liu,
- Yanlin Liu,
- Huaping Tang,
- Huaping Tang,
- Qiang Xu,
- Qiang Xu,
- Mei Deng,
- Mei Deng,
- Qiantao Jiang,
- Qiantao Jiang,
- Guoyue Chen,
- Guoyue Chen,
- Yuanying Peng,
- Yuanying Peng,
- Yunfeng Jiang,
- Yunfeng Jiang,
- Yun Jiang,
- Yuming Wei,
- Yuming Wei,
- Youliang Zheng,
- Xiujin Lan,
- Xiujin Lan,
- Jian Ma,
- Jian Ma
Affiliations
- Huangxin Chen
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Huangxin Chen
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Conghao Zhao
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Conghao Zhao
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yaoyao Yang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yaoyao Yang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Zhaoyong Zeng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Zhaoyong Zeng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Wei Li
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Wei Li
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yanlin Liu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yanlin Liu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Huaping Tang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Huaping Tang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Qiang Xu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Qiang Xu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Mei Deng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Mei Deng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Qiantao Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Qiantao Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Guoyue Chen
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Guoyue Chen
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yuanying Peng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yuanying Peng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yunfeng Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yunfeng Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yun Jiang
- Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, China
- Yuming Wei
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yuming Wei
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Youliang Zheng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Xiujin Lan
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Xiujin Lan
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jian Ma
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jian Ma
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fpls.2022.999414
- Journal volume & issue
-
Vol. 13
Abstract
Maximum root length (MRL) plays an important role in the uptake of nutrients and resisting abiotic stresses. Understanding the genetic mechanism of root development is of great significance for genetic improvement of wheat. Previous studies have confirmed that parental reproductive environment (PRE) has a significant impact on growth and development of the next generation in the whole life cycle of a given plant. In this study, a recombinant inbred line population genotyped using the Wheat55K SNP array, was used to map quantitative trait loci (QTL) for wheat seedling MRL based on the harvested seeds from five different PREs. A total of 5 QTL located on chromosomes 3D and 7A were identified. Among them, QMrl.sicau-2SY-3D.2 located in a 4.0 cM interval on chromosome 3D was likely independent of PREs. QMrl.sicau-2SY-7A.2 was detected in two tests and probably influenced by PREs. The effect of QMrl.sicau-2SY-3D.2 was further validated using the tightly linked kompetitive allele specific PCR (KASP) marker, KASP-AX-111589572, in populations with different genetic backgrounds. Lines with a combination of positive alleles from QMrl.sicau-2SY-3D.2 and QMrl.sicau-2SY-7A.2 have significantly longer MRL. Furthermore, four genes (TraesCS3D03G0612000, TraesCS3D03G0608400, TraesCS3D03G0613600, and TraesCS3D03G0602400) mainly expressed in wheat root were predicted to be associated with root growth. Taken together, this study reports on a major QTL independent of PREs and lays a foundation for understanding the regulation mechanism of wheat MRL at the seedling stage.
Keywords
- maximum root length
- quantitative trait loci
- wheat
- Wheat55K SNP array
- parental reproductive environment
