Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples

Kędrak-Jabłońska Agnieszka; Budniak Sylwia; Szczawińska Anna; Reksa Monika; Krupa Marek; Szulowski Krzysztof

doi:10.2478/jvetres-2018-0075

Journal of Veterinary Research (Dec 2018)

Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples

Kędrak-Jabłońska Agnieszka,
Budniak Sylwia,
Szczawińska Anna,
Reksa Monika,
Krupa Marek,
Szulowski Krzysztof

Affiliations

Kędrak-Jabłońska Agnieszka: Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, Poland
Budniak Sylwia: Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, Poland
Szczawińska Anna: Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, Poland
Reksa Monika: Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, Poland
Krupa Marek: Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, Poland
Szulowski Krzysztof: Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, Poland

DOI: https://doi.org/10.2478/jvetres-2018-0075
Journal volume & issue: Vol. 62, no. 4
pp. 549 – 554

Abstract

Read online

Introduction: The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three Bacillus anthracis genes in contaminated liver and blood samples. The goals for detection were rpoB gene as a chromosomal marker, pag gene located on plasmid pXO1, and capC gene located on plasmid pXO2.

Published in Journal of Veterinary Research

ISSN: 2450-8608 (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://sciendo.com/journal/JVETRES

About the journal

Abstract

Keywords