Determination of the Bioactive Compounds from <i>Echinacea purpurea</i> (L.) Moench Leaves Extracts in Correlation with the Antimicrobial Activity and the In Vitro Wound Healing Potential
Cristina Burlou-Nagy,
Florin Bănică,
Rodica Anamaria Negrean,
Tünde Jurca,
Laura Grațiela Vicaș,
Eleonora Marian,
Ildikó Bácskay,
Rita Kiss,
Pálma Fehér,
Simona Ioana Vicaș,
Florina Miere (Groza),
Adriana Ramona Memete,
Annamaria Pallag
Affiliations
Cristina Burlou-Nagy
Doctoral School of Biomedical Sciences, University of Oradea, Universitatii Street, 1, 410087 Oradea, Romania
Florin Bănică
Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania
Rodica Anamaria Negrean
Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, 1st December Square 10, 410068 Oradea, Romania
Tünde Jurca
Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania
Laura Grațiela Vicaș
Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania
Eleonora Marian
Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania
Ildikó Bácskay
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, Hungary
Rita Kiss
Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, Hungary
Pálma Fehér
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, Hungary
Simona Ioana Vicaș
Department of Food Engineering, Faculty of Environmental Protection, University of Oradea, No. 26 General Magheru Avenue, 410087 Oradea, Romania
Florina Miere (Groza)
Department of Preclinical Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, 1st December Square 10, 410068 Oradea, Romania
Adriana Ramona Memete
Department of Food Engineering, Faculty of Environmental Protection, University of Oradea, No. 26 General Magheru Avenue, 410087 Oradea, Romania
Annamaria Pallag
Doctoral School of Biomedical Sciences, University of Oradea, Universitatii Street, 1, 410087 Oradea, Romania
This study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study’s objective was to assess the bioactive components (total phenol and flavonoid content) and antioxidant activity of EPF extracts using the DPPH test method. The antioxidant capacity and the quantities of the compounds with antioxidant capacity were evaluated by spectrophotometric methods. Antimicrobial activity has been investigated against various pathogenic microorganisms. The minimum inhibitory concentration was determined by the microdilution method. Additionally, our work used a scratch test to examine the in vitro wound healing effects of EPF extract on NHDF cells. Statistical analysis was used to quantify the rate of migration and proliferation of fibroblast cells within the wound. Microscope pictures of fibroblast cells exposed to various EPF extract dosages were processed to estimate the width of the wound, area of the wound, and cell density inside the wound. The study proved that there was a relationship between the antioxidant, antimicrobial, and wound healing ability of EPF extracts.
