GigaByte (Apr 2022)
Annotation of putative circadian rhythm-associated genes in Diaphorina citri (Hemiptera: Liviidae)
- Max Reynolds ,
- Lucas de Oliveira ,
- Chad Vosburg ,
- Thomson Paris ,
- Crissy Massimino ,
- Jordan Norus ,
- Yasmin Ortiz ,
- Michelle Espino ,
- Nina Davis ,
- Ron Masse ,
- Alan Neiman ,
- Rachel Holcomb ,
- Kylie Gervais ,
- Melissa Kemp ,
- Maria Hoang ,
- Teresa D. Shippy ,
- Prashant S. Hosmani ,
- Mirella Flores-Gonzalez ,
- Kirsten Pelz-Stelinski ,
- Jawwad A. Qureshi ,
- Lukas A. Mueller ,
- Wayne B. Hunter ,
- Joshua B. Benoit ,
- Susan J. Brown ,
- Tom D’Elia ,
- Surya Saha
Affiliations
- Max Reynolds
- Indian River State College, Fort Pierce, FL 34981, USA
- Lucas de Oliveira
- ORCiD
- Indian River State College, Fort Pierce, FL 34981, USA
- Chad Vosburg
- ORCiD
- Indian River State College, Fort Pierce, FL 34981, USA, Department of Plant Pathology and Environmental Microbiology, Pennsylvania State University, University Park, PA 16802, USA
- Thomson Paris
- ORCiD
- Entomology and Nematology Department, University of Florida, North Florida Research and Education Center, Research Road, Quincy 32351, Florida, USA
- Crissy Massimino
- ORCiD
- Indian River State College, Fort Pierce, FL 34981, USA
- Jordan Norus
- Indian River State College, Fort Pierce, FL 34981, USA
- Yasmin Ortiz
- ORCiD
- Indian River State College, Fort Pierce, FL 34981, USA
- Michelle Espino
- Indian River State College, Fort Pierce, FL 34981, USA
- Nina Davis
- Indian River State College, Fort Pierce, FL 34981, USA
- Ron Masse
- Indian River State College, Fort Pierce, FL 34981, USA
- Alan Neiman
- Indian River State College, Fort Pierce, FL 34981, USA
- Rachel Holcomb
- Indian River State College, Fort Pierce, FL 34981, USA
- Kylie Gervais
- Indian River State College, Fort Pierce, FL 34981, USA
- Melissa Kemp
- Indian River State College, Fort Pierce, FL 34981, USA
- Maria Hoang
- Indian River State College, Fort Pierce, FL 34981, USA
- Teresa D. Shippy
- ORCiD
- Division of Biology, Kansas State University, Manhattan, KS 66506, USA
- Prashant S. Hosmani
- ORCiD
- Boyce Thompson Institute, Ithaca, NY 14853, USA
- Mirella Flores-Gonzalez
- ORCiD
- Boyce Thompson Institute, Ithaca, NY 14853, USA
- Kirsten Pelz-Stelinski
- Department of Entomology and Nematology, University of Florida, Lake Alfred, FL 33850, USA
- Jawwad A. Qureshi
- Indian River Research and Education Center, University of Florida, IFAS, 2199 South Rock Road, Fort Pierce, FL 34945-3138, USA, Southwest Florida Research and Education Center, University of Florida, IFAS, 2685 State Road 29 North, Immokalee, FL 34142, USA
- Lukas A. Mueller
- ORCiD
- Boyce Thompson Institute, Ithaca, NY 14853, USA
- Wayne B. Hunter
- ORCiD
- USDA-ARS, US Horticultural Research Laboratory, Fort Pierce, FL 34945, USA
- Joshua B. Benoit
- ORCiD
- Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA
- Susan J. Brown
- ORCiD
- Division of Biology, Kansas State University, Manhattan, KS 66506, USA
- Tom D’Elia
- Indian River State College, Fort Pierce, FL 34981, USA
- Surya Saha
- ORCiD
- Boyce Thompson Institute, Ithaca, NY 14853, USA, Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USA
Abstract
The circadian rhythm involves multiple genes that generate an internal molecular clock, allowing organisms to anticipate environmental conditions produced by the Earth’s rotation on its axis. Here, we present the results of the manual curation of 27 genes that are associated with circadian rhythm in the genome of Diaphorina citri, the Asian citrus psyllid. This insect is the vector for the bacterial pathogen Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus greening disease (Huanglongbing). This disease severely affects citrus industries and has drastically decreased crop yields worldwide. Based on cry1 and cry2 identified in the psyllid genome, D. citri likely possesses a circadian model similar to the lepidopteran butterfly, Danaus plexippus. Manual annotation will improve the quality of circadian rhythm gene models, allowing the future development of molecular therapeutics, such as RNA interference or antisense technologies, to target these genes to disrupt the psyllid biology.
