The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions

Abstract

Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observed