Methodology established for the detection of circulating tumor DNA by hybridization capture
Xumei Yao,
Zhilin Zhou,
Yuancai Xie,
Zhigang Huang,
Shixin Lu,
Chaoyu Liu,
Jun Wang,
Xiaohua Li
Affiliations
Xumei Yao
1Department of Research & Development, Shenzhen Shiningbiotek Co., Ltd., Shenzhen, Guangdong, 518053, China
Zhilin Zhou
2Department of General Surgery, Liyuan Hospital, Tongji, Medical College, Huazhong University of Science & Technology, Wuhan, Hubei, 430077, China
Yuancai Xie
3Department of Thoracic Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, Province, China
Zhigang Huang
4Department of General Practice, Peking University Shenzhen Hospital, Shenzhen, Guangdong, 518036, Province, China
Shixin Lu
1Department of Research & Development, Shenzhen Shiningbiotek Co., Ltd., Shenzhen, Guangdong, 518053, China
Chaoyu Liu
1Department of Research & Development, Shenzhen Shiningbiotek Co., Ltd., Shenzhen, Guangdong, 518053, China
Jun Wang
5Shenzhen Key Laboratory of Microbial Genetic Engineering, Vascular Disease Research Center, College of Life Sciences & Oceanography, Guangdong Provincial Key Laboratory of Regional Immunity & Disease, Carson International Cancer Center, School of Medicine, Shenzhen University, Shenzhen, Guangdong, 518060, China
Xiaohua Li
1Department of Research & Development, Shenzhen Shiningbiotek Co., Ltd., Shenzhen, Guangdong, 518053, China
Roche's AVENIO ctDNA analysis kits and bioinformatics analysis (the AVENIO system) are accessible to all NGS laboratories. We have developed an approach, namely the Sec-Seq system, and compared the accuracy, sensitivity, repeatability and economic cost between the AVENIO system and the Sec-Seq system. Both methods share the comparable accuracy and sensitivity in detecting the variant allele frequency of 0.0005, while the Sec-Seq system shows better accuracy in detecting the variant allele frequency of 0.001. Furthermore, the Sec-Seq system displays a much better detection sensitivity than the AVENIO system. The Sec-Seq system has satisfactory performance in detecting the rare genetic variants in ctDNA with lower economic cost compared with the AVENIO system.
