Effect of sulfate availability on root traits and microRNA395 expression in sugar beet

Abstract

Nutritional stress is one of the main limits to sugar beet yield. This study evaluated morphological and molecular responses of sugar beet to changes in sulfate availability. Morphological characteristics of the root system and the accumulation of microRNA395 (miR395) were examined in sulfate(S)-supplemented and S-deprived seedlings under hydroponic conditions. We also investigated the functional role of miR395 in regulating the expression of APS1 gene coding for ATP-sulfurylase in roots and leaves. The S-deprived seedlings showed a significant increase in the number of root tips, in the miR395 expression in leaves but not in roots, and in the expression of APS1 gene. Our results indicate that miR395 may be a useful biomarker for sulfate status in sugar beet.

Keywords

• atp-sulfurylase
• beta vulgaris
• root morphology