PKM2 Expression and Elevated CD163 in Macrophage Synergistically Promote Osteosarcoma Progression

Abstract

Objective To investigate the relation between PKM2 and related pathological features of osteosarcoma, to further explore the markers for predicting the prognosis of osteosarcoma patients. Methods We collected 88 cases of paraffin-embedded primary osteosarcoma specimens, and the specimens were constructed by tissue microarray. Serial sections were used to detect the expression of PKM2 in the above tissues by immunohistochemistry. Kaplan-Meier analysis was used to demonstrate the prognostic value. Correlation analysis between parameters and patient's prognosis was performed using Cox regression model. Results M2 macrophage-specific marker CD163 was elevated in osteosarcoma matrix, suggesting that M2 macrophages were densely infiltrated in osteosarcoma. PKM2 overexpression is positively correlated with M2 macrophage infiltration in osteosarcoma. Conclusion PKM2 overexpression and M2 macrophage infiltration were influence factors for patients' poor prognosis.

Keywords

• osteosarcoma
• m2 macrophages
• pkm2
• immunohistochemistry