Alginate-gelatin based core-shell capsule enhances the osteogenic potential of human osteoblast-like MG-63 cells

Cherng-Jyh Ke; Kuo-Hui Chiu; Ching-Yun Chen; Chiung-Hua Huang; Chun-Hsu Yao

Materials & Design (Nov 2021)

Alginate-gelatin based core-shell capsule enhances the osteogenic potential of human osteoblast-like MG-63 cells

Cherng-Jyh Ke,
Kuo-Hui Chiu,
Ching-Yun Chen,
Chiung-Hua Huang,
Chun-Hsu Yao

Affiliations

Cherng-Jyh Ke: Biomaterials Translational Research Center, China Medical University Hospital, No. 2, Yude Rd., Taichung City 40447, Taiwan
Kuo-Hui Chiu: Department of Biological Science and Technology, China Medical University, No. 100, Sec. 1, Jingmao Rd., Taichung City 406040, Taiwan
Ching-Yun Chen: Department of Biomedical Sciences and Engineering, National Central University, Taoyuan 32001, Taiwan
Chiung-Hua Huang: Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan; Corresponding authors at: Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40202, Taiwan (C.-H. Yao). Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan (C.-H. Huang).
Chun-Hsu Yao: Biomaterials Translational Research Center, China Medical University Hospital, No. 2, Yude Rd., Taichung City 40447, Taiwan; Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40202, Taiwan; School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Biomedical Informatics, Asia University, Taichung 41354, Taiwan; Corresponding authors at: Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40202, Taiwan (C.-H. Yao). Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan (C.-H. Huang).

Journal volume & issue: Vol. 210
p. 110109

Abstract

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Three-dimensional (3D) cell culture enhanced cells-cells communication, promoting developmental processes and influencing cell performance. In this study, 3D alginate-gelatin core–shell capsules (AGC) were fabricated by using a microfluidic device. Human osteoblast-like MG-63 cells were encapsulated within the 3D model. The morphology and physicochemical properties of the AGC were characterized by using scanning electron microscopy (SEM), Flourier Transform Infrared Spectrum (FTIR), Gravimetric thermal analyzer (TGA). The mRNA levels of osteogenetic gene markers were measured by quantitative real-time RT-PCR. The result of a 9.6% weight loss after a 7-day soaking indicates that the AGC exhibited excellent stability. The encapsulated MG-63 cells formed clusters alongside the core of AGC and obtained 200% cell viability after 21-day long-term cultivation. Compared to traditional 2D cell culture, the 3D cultured MG-63 cells performed higher alkaline phosphatase (ALP), a 9.3-fold increase of calcium mineralization activity, and enhanced osteogenic gene expression of BGLAP, COL1A1, and Runx2. These observations suggested that AGC obtains the potential as an ideal 3D microenvironmental cell culture system for biomedical application.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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