A new biosensor detection system to overcome the Debye screening effect: dialysis-silicon nanowire field effect transistor

Abstract

Hang Chen, Xiaoqian Zhao, Zhong Xi, Ye Zhang, Hang Li, Zengyao Li, Haoze Shi, Longchang Huang, Renhui Shen, Jianxin Tao, Tong WangDepartment of General Surgery, Nanjing Medical University Affiliated Wuxi People’s Hospital, Wuxi, Jiangsu Province, People’s Republic of ChinaBackground: A silicon nanowire field effect transistor biosensor has four advantages in the detection of small biomolecules. It is mark-free, immediately responsive, highly sensitive, and specific. However, because of environments with a high salt concentration, the Debye screening effect has been a major issue in biological detection.Objective: To overcome Debye screening effect, realize the clinical application of silicon nanowire field effect transistor and verify its specificity and sensitivity.Materials and methods: The test solution was desalted by miniature blood dialyzer, and then the tumor markers were detected by silicon nanowire field effect transistor.Results: Tumor markers in serum were detected successfully and their sensitivity and specificity were verified.Conclusion: This method was found to effectively promote the development of semiconductor materials in biological solution detection.Keywords: silicon nanowire, biosensor, Debye screening effect, tumor marker, dialysis

Keywords

• silicon nanowire
• biosensor
• Debye screening effect
• tumor marker
• dialysis