Nature Communications (May 2016)

Highly efficient baculovirus-mediated multigene delivery in primary cells

  • Maysam Mansouri,
  • Itxaso Bellon-Echeverria,
  • Aurélien Rizk,
  • Zahra Ehsaei,
  • Chiara Cianciolo Cosentino,
  • Catarina S. Silva,
  • Ye Xie,
  • Frederick M. Boyce,
  • M. Wayne Davis,
  • Stephan C. F. Neuhauss,
  • Verdon Taylor,
  • Kurt Ballmer-Hofer,
  • Imre Berger,
  • Philipp Berger

DOI
https://doi.org/10.1038/ncomms11529
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 13

Abstract

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Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multiprime for genome engineering by CRISPR/Cas9.