Armaghane Danesh Bimonthly Journal (Oct 2013)
Effects Of Anethum graveolens L. Etheric Fraction Of Ethanol And Aqueous Seeds Extracts On Sex Hormones And Histomorphometrical Changes Of Female Rat Reproductive System
Abstract
Abstract Background & aim: Previous research confirmed the effects of ethanol and aqueous seed extracts on female reproductive system. The aim of this study was to evaluate the effect of alcohol extract of ether and aqueous fractions of dill seed histomorphometric changes in sex hormones and the reproductive system of female rats. Materials & Methods: The present experimental study was conducted on 35 female rats that were in estrus phase. Rats were divided into 5 groups, the control, low dose and high-ether fraction of aqueous extract 0.5 and 5 g/kg and fractions with high and low doses of alcohol0 .045 and 0.45 g/ kg respectively. The experimental group received 1 ml of mentioned dose and the control group received 1 ml distilled water daily for a period of 10 days. At the end of the experiment, the rats in the estrus phase were anesthetized and blood samples were taken from the dorsal aorta for more investigations. Ovaries and uterus tissue sections were prepared and stained with hematoxylin - eosin and Masson tri-chrome for morphometry by light microscopy. Levels of progesterone and estradiol levels were measured by radioimmunoassay and ELISA. Data were analyzed by one-way ANOVA. Results: Serum levels of progesterone and estradiol groups showed no significant difference between the experimental and the control group (P>0.05). In addition, the endometrial and myometrial thickness of uterus statistically decreased in high dose of etheric fraction of the aqueous extract (P<0.05). Conclusion: Oral administration of ether fraction of aqueous and alcoholic extracts of plant seeds will not be able to hormonal changes, as well as the other hormone-related variables. Key words: Dill seed, Estrogen, Progesterone, Etheric fraction, Reproductive system
