مجله دانشکده پزشکی اصفهان (Jul 2014)
Effect of Low-Power Laser on Wound Healing and Blood Sugar in Diabetic Rats
Abstract
Background: According to the World Health Organization (WHO) reports, the number of patients with diabetes will be doubled by 2050 to reach 250 million. This study aimed to assess the effect of low-power laser, with power of 5 mW, wavelength of 632.8 nm and energy of 4 j/cm2, on wound healing and blood sugar of normal and diabetic rats. Methods: 36 male rats, weighing 200 to 250 g, were divided into 4 groups including 9 animals in each group. Groups I and II were considered as two control groups. Group I was the control group without skin wound (normal control) and group II with skin wound (sham operated) treated using laser. Group III was considered as diabetic with skin wound, and group IV with skin wound treated by low-power laser with a wavelength of 632.8 nm and a power of 5 mW. In the time scales of 4, 7 and 10 days after surgery, the wound area was measured to calculate wound healing. Blood sugar levels and urine hydroxyproline were measured via colorimeter. Findings: The wound healing in the 7th day of treatment in both second (healthy laser) and forth (diabetic laser) group was significant in comparison with the control group (P < 0.05 and P < 0.01, respectively). On the 10th day, wound healing showed significant difference in the mentioned two groups (P < .0.05 for both) and third group (P < 0.01) in comparison with the control group. Conclusion: The laser treatment in the wavelength of 632.8 nm, power of 5 mW and energy of 4 j/cm2 showed significant effect on blood sugar levels and wound healing in a time-dependent manner in both normal and diabetic rats. Laser healing effects was confirmed using both histological and biochemical studies on hydroxyproline concentration in the treated groups.
