Bioactivities of a New Pyrrolidine Alkaloid from the Root Barks of Orixa japonica
Xin Chao Liu,
Daowan Lai,
Qi Zhi Liu,
Ligang Zhou,
Qiyong Liu,
Zhi Long Liu
Affiliations
Xin Chao Liu
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China
Daowan Lai
Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100193, China
Qi Zhi Liu
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China
Ligang Zhou
Department of Plant Pathology, China Agricultural University, Haidian District, Beijing 100193, China
Qiyong Liu
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center of Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhi Long Liu
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China
A new pyrrolidine alkaloid named (Z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one was isolated from the ethanol extract of the root barks of Orixa japonica. The structure of the new alkaloid was elucidated on the basis of NMR and MS analysis. The compound exhibited larvicidal activity against the fourth instar larvae of Aedes aegypti (LC50 = 232.09 μg/mL), Anopheles sinensis (LC50 = 49.91 μg/mL), and Culex pipiens pallens (LC50 = 161.10 μg/mL). The new alkaloid also possessed nematicidal activity against Bursaphelenchus xylophilus (LC50 = 391.50 μg/mL) and Meloidogynein congnita (LC50 = 134.51 μg/mL). The results indicate that the crude ethanol extract of O. japonica root barks and its isolated pyrrolidine alkaloid have potential for development into natural larvicides and nematicides.
