Bangladesh Journal of Pharmacology (Sep 2008)

Antiamoebic activity of marine sponge Haliclona exigua (Krikpatrick)

  • V. Lakshmi,
  • A. Saxena,
  • S. K. Mishra,
  • M. Mishra,
  • S. Srivastava,
  • S. Ghoshal

DOI
https://doi.org/10.3329/bjp.v4i1.1083
Journal volume & issue
Vol. 4, no. 1

Abstract

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The methanol and methanol-chloroform (1:1) extracts of the freshly collected Haliclona exigua showed minumim inhibitory concentration (MIC) of 125 ?g/mL and 250 ?g/mL respectively in in vitro studies, but when both of these were tested in vivo in rats, only methanol-chloroform showed 80% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entameba histolytica. Therefore only methanol-chloroform extract was further fractionated into four fractions (n-hexane, chloroform, n-butanol soluble and n-butanol insoluble fractions). Out of these, only n-hexane and n-butanol soluble fractions showed 80% inhibition of trophozoites at 900 mg/kg dose. Further the chromatography of the n-butanol fraction yielded araguspongin-C which showed promising results at different doses.

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