QTL mapping for microtuber dormancy and GA3 content in a diploid potato population
Raja Mohib Muazzam Naz,
Mengtai Li,
Safia Ramzan,
Gege Li,
Jun Liu,
Xingkui Cai,
Conghua Xie
Affiliations
Raja Mohib Muazzam Naz
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Mengtai Li
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Safia Ramzan
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Gege Li
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Jun Liu
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Xingkui Cai
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
Conghua Xie
National Center for Vegetable Improvement (Central China), Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China
The genetic control of dormancy is poorly understood in most plant species, but dormancy is a prominent feature for the potato industry. We used the microtuber system, in which tubers were produced in vitro and stored at 20°C, to perform quantitative trait locus (QTL) analysis for dormancy and gibberellic acid (GA3) content in an F1 population consisting of 178 genotypes derived from an interspecific cross between Solanum chacoense acc. PI 320285 (long dormancy) and Solanum phureja acc. DM1-3 516 R44 (short dormancy). In this analysis, 163 markers were used to construct a genetic map with a total length of 591.8 cM. Through QTL analysis, we identified 22 markers closely linked to the timing of dormancy release and GA3 content. The male parent alleles were closely related with long dormancy, with the most significant effect on chromosome I, which accounted for 9.4% of phenotypic variation. The dormancy and GA3 QTLs localized to the same position in the genome, confirming that same genomic region controls GA3 content at different developmental stages or in dormant and sprouting tubers. The identified QTLs may be useful for future breeding strategies and studies of dormancy in potato.
