Association of angiotensin converting enzyme (ACE), D/I polymorphisms with recurrent pregnancy loss

Abstract

Background & aim: Recurrent pregnancy loss (RPL) refers to the occurrence of two or more consecutive losses of clinically recognized pregnancies prior to the 20th week of gestation. The aim of this study was to investigate the hypothesis that recurrent pregnancy loss (RPL) is associated with a common insertion-deletion polymorphism in the angiotensin-converting enzyme gene.. Methods: The present paper intended to study the ACE deletion (D)/insertion (I) polymorphism in women with recurrent abortion. One hundred patients with recurrent abortions (at least two) as cases and one hundred healthy female with two or more normal term deliveries and without a history of abortion selected as controls. Genomic DNA was isolated from peripheral blood leukocytes and the ACE insertion/deletion (I/D) polymorphism in intron 16 was performed by polymerase chain reaction (PCR). For the statistical analysis, SPSS software version18 was used and Chi-square tests were calculated. Results: Seven patients, (7 %), and non from the control group, were homozygote (I/I) for ACE polymorphism (OR=22.82 95% CI=1.26-412.69 p=0.034), depicting no significant associations between ACE D allele or DD genotype and RPL. Conclusion: The present study showed no significant associations between ACE D allele or DD genotype and RPL.

Keywords

• Recurrent Pregnancy Loss
• Angiotensin Converting Enzyme
• Polymerase