Association between single nucleotide polymorphisms of F3 gene (rs12029080 T>G and rs11165176 C>T) with thrombosis in SARS-CoV-2 patients in Babylon Province

Abstract

Background: Severe acute respiratory syndrome coronavirus 2 is a respiratory virus that has a strong association with pulmonary morbidity and thrombosis and has caused death in much of the sick population. The coagulation factor III gene (F3) produces tissue factor (TF) a cell-surface glycoprotein. It is the primary initiator of the extrinsic blood coagulation cascade. Objectives: The study aimed to identify the single nucleotide polymorphism (SNP) of F3 gene (rs12029080 T>G and rs11165176 C>T) and D-dimer linked to thrombosis in coronavirus disease (COVID-19) patients. Materials and Methods: Blood samples were collected from 30 patients infected by COVID-19 in Babylon Province. Detection of the polymorphism was done by using sequencing technique. Results: The results of the polymerase chain reaction of the amplification targeted region had shown two valid SNPs: rs11165176C>T and rs11165176. We discovered that heterozygous C/T had a higher genotypic frequency than homozygous C/C (odd ratio (OR) = 1.14, 95% confidence interval (CI) = 0.41–3.15, P = 0.796) and the homozygous variation T/T genotypic frequency is comparable to that of homozygous C/C (OR = 1.4, 95% CI = 0.43–4.86, P = 0.543). The C and T alleles frequencies showed statistical difference as reported in F3 (rs11165176) between COVID-19 patients and control (P = 0.053), 29 (48.3%) and 31 (51.6%), respectively, than in control groups, C allele 34 (56.7%) and T allele 26 (43.3%). Therefore, the T allele was most frequent in COVID-19 patients and control groups. Conclusion: According to the results of our statistical research, there was a significant link between F3 gene rs11165176 and the development of thrombosis in patients with COVID-19 to be a potential D-dimer associated with COVID-19 patients.

Keywords

• covid-19 patients
• d-dimer
• f3 gene
• snps