Low Concentration of Rotenone Impairs Membrane Function of <i>Spodoptera litura</i> Cells by Promoting Their Aggregation

Abstract

Rotenone is a widely used botanical insecticide, which can inhibit the mitochondrial respiratory chain of various insect cells, while the mechanism of its toxicity to insect cells needs further investigation. The purpose of this study was to understand the toxicity level of low (0.2 µg/mL) and high (20 µg/mL) concentrations of rotenone in Spodoptera litura cells (SL-1) using trypan blue and Coomassie brilliant blue staining assays. Our study found that rotenone possessed cytotoxicity against SL-1 cells with varying effects of action between high and low concentrations. After low-concentration rotenone treatment, the SL-1 cells showed obvious aggregation time-dependently, with the fastest aggregation rate observed under the first 8 h of treatment time, but no such phenomenon was observed at high concentration. Furthermore, this aggregation phenomenon caused SL-1 cells to squeeze each other and led to the destruction of the cell membrane structure and function. Taken together, the results suggested that treatment with a low concentration of rotenone exhibited a chronic toxic effect that was significantly different from treatment with a high concentration of rotenone, which provides new insight into the cytotoxic mechanism of rotenone.

Keywords

• botanical insecticide
• cytotoxicity
• membrane structure
• ovarian cell
• pest management