Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity

Lidiya Lisitskaya; Yeonoh Shin; Aleksei Agapov; Anna Olina; Ekaterina Kropocheva; Sergei Ryazansky; Alexei A. Aravin; Daria Esyunina; Katsuhiko S. Murakami; Andrey Kulbachinskiy

doi:10.1038/s41467-022-32079-5

Nature Communications (Aug 2022)

Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity

Lidiya Lisitskaya,
Yeonoh Shin,
Aleksei Agapov,
Anna Olina,
Ekaterina Kropocheva,
Sergei Ryazansky,
Alexei A. Aravin,
Daria Esyunina,
Katsuhiko S. Murakami,
Andrey Kulbachinskiy

Affiliations

Lidiya Lisitskaya: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”
Yeonoh Shin: Department of Biochemistry and Molecular Biology, Pennsylvania State University
Aleksei Agapov: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”
Anna Olina: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”
Ekaterina Kropocheva: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”
Sergei Ryazansky: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”
Alexei A. Aravin: Division of Biology and Biological Engineering, California Institute of Technology
Daria Esyunina: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”
Katsuhiko S. Murakami: Department of Biochemistry and Molecular Biology, Pennsylvania State University
Andrey Kulbachinskiy: Institute of Molecular Genetics, National Research Center “Kurchatov Institute”

DOI: https://doi.org/10.1038/s41467-022-32079-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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The authors describe programmable Argonaute nucleases that use small DNA guides to recognize and cleave RNA targets and can sense mismatches and modifications in RNA targets, making them potential tools for RNA biotechnology.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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