Molecular detection and genotyping of HMPV in patients with severe acute respiratory infection in India

Abstract

Background Human metapneumovirus (HMPV) is a common respiratory pathogen that causes respiratory tract infections. In India, HMPV has been identified as one of the leading causes of morbidity and mortality in infants and young children with respiratory tract infections. The most reported sublineages of HMPV in India are B1, B2, A2b and A2c.Objective A retrospective study was conducted to determine the circulating genotypes of HMPV among SARI cases from January 2016 to December 2018.Materials and methods Positive throat swab samples were confirmed with real-time RT–PCR. Subsequently, these samples were analysed using semi-nested conventional RT–PCR targeting the G gene, followed by sequencing and phylogenetic analysis. Clinical data analysis was also performed using SPSS 15.0 software.Results All 20 samples from the SARI cases were classified under the A2c sublineage of HMPV. Phylogenetic analysis indicated that these strains were genetically related to those circulating in Japan, China, and Croatia. Among the samples, ten showed 111-nucleotide duplications, while the other ten had 180-nucleotide duplications.Conclusion Clinical analysis showed that four cases had coinfections with other pathogens. Our extensive analysis of patient samples determined that HMPV, especially the A2c genotype, significantly contributed to SARI cases within our study population, which signifies the importance of considering HMPV as a probable aetiological agent when investigating SARI outbreaks.

Keywords

• HMPV
• SARI
• RT–PCR
• climate action
• good health and well-being