Sugarcane leaf polysaccharide exerts a therapeutic effect on cardiovascular diseases through necroptosis
Kaili Sun,
Renyikun Yuan,
Jia He,
Youqiong Zhuo,
Ming Yang,
Erwei Hao,
Xiaotao Hou,
Chun Yao,
Shilin Yang,
Hongwei Gao
Affiliations
Kaili Sun
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China
Renyikun Yuan
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China
Jia He
State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China
Youqiong Zhuo
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China
Ming Yang
State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China
Erwei Hao
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica/Guangxi University of Chinese Medicine, Nanning, Guangxi, 530001, China; Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues/Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China
Xiaotao Hou
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica/Guangxi University of Chinese Medicine, Nanning, Guangxi, 530001, China; Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues/Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China
Chun Yao
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China; Corresponding author.
Shilin Yang
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China
Hongwei Gao
College of Pharmacy, Guangxi University of Chinese Medicine, Nanning, 530000, China; Corresponding author.
Background: Necroptosis, a novel form of programmed cell death wherein the necrotic morphology is characterized by swelling of the cells, rupture of the plasma membrane, and dysfunction of the organelle, has been always observed in cardiovascular diseases. Sugarcane leaf polysaccharide (SLP) are primary components present in sugarcane leaves that exert cardiovascular protective effects. However, the positive effect of SLP and underlying mechanisms in myocardial ischemia-reperfusion (MI/R) remain unexplored. Aim: In this study, the protective effects of SLP on MI/R injury were investigated under in vitro and in vivo conditions. Methods: The protective effects of SLP on MI/R injury were assessed using tertiary butyl hydrogen peroxide (TBHP)–stimulated-H9c2 cells in the in vitro assay and using Sprague Dawley rats in the in vivo assay. Results: In vitro, SLP significantly reversed TBHP-induced H9c2 cell death by inhibiting necroptosis and oxidative stress. SLP exerted antioxidant activity through the Nrf2/HO-1 pathway. SLP suppressed necroptosis by decreasing phosphorylation of RIP1, RIP3, and MLKL in TBHP-stimulated H9c2 cells. In vivo, SLP attenuated MI/R injury by decreasing the myocardial infarct area; increasing myeloperoxidase and superoxide dismutase levels; and reducing malondialdehyde, interleukin-6, and tumor necrosis factor-α levels.
