PENGARUH IODIUM DAN SELENIUM TERHADAP STATUS TIROID DAN AKTIVITAS GLUTHATION PEROKSIDASE TIKUS WISTAR JANTAN HIPOTIROID

Abstract

Background. Iodine and selenium deficiency have important role on hypothyroidism.Iodine is an essential microelement in the thyroid hormone in its role on developmentand growth. Selenium is an essential component of Glutathione Peroxidises (GSHPx)the antioxidant enzyme. Objective. The aim of this study was to evaluate thyroidstatus by measuring Thyroid Stimulating Hormone (TSH) and activity of GSH-Pxafter iodine and selenium treatment. Method. This is an experimental, randomizedpre and post control study. Twenty five weeks male Wistar rats were induced bypropylthiouracil (PTU) for four week before and four week during interventions.Subjects were divided into four groups offive. Group I (control hypothyroidism),group II (iodine supplementation), group III (selenium supplementation), group IV(iodine + selenium supplementation). Data were analyzed using ANOVA and pairedt test at 95 percent confidence interval. Results. There is no difference betweenLevels of TSH in hypothyroidism between groups (p=0.721). Levels and Delta ofTSH between groups were difference at the end study, with p=0.013 and p=0.000respectivelly. Levels of TSH before and after treatment were different in each group,(group I, p=0.031, group II, p=0.003, group III, p=0.004, group IV, p=0.010). Therewere no different activity of GSH-Px between groups and in each group p=0.081and p=0.104. Delta of GSH-Px was not different, p=0.476. Activity of GSH-Px beforeand after treatment only showed differences in group IV (group I, p=0.042, group II,p=0.009, group III, p=0.01, group IV, p=0.623). Conclusion. Intervention of iodineand selenium can reduce levels of TSH and GSH-Px activity.Keywords: GSH-Px, hypothyroidism, iodine, selenium, TSH