Офтальмохирургия (Dec 2015)
REPARATIVE REGENERATION OF CORNEA AT NANOSTRUCTURED BIOPOLYMER OF HYALURONIC ACID APPLICATION
Abstract
Purpose. Estimation of corneal reparative regeneration processes course at application of bioplastic material – nanostructured biopolymer of hyaluronic acid on the model of chemical (alkaline and acid) cornea burn.Material and methods. Experimental modeling of cornea chemical burn was carried out on 36 rabbits (72 eyes). The study had two series of cornea burn: alkaline (18 rabbits – 36 eyes) and acid (18 rabbits – 36 eyes) corneal burns. In each of the series there was identified: an experimental group, which used the applique of bioplastic material «hyamatrix» according to the method of prof. V.N. Kanyukov and control one with Solcoseryl instillations. The clinical study included an examination of the eye anterior segment using the focus and side lighting and photographic recording. At the of period of 3, 7, 14, 30 and 90 days the animals were removed from the experiment for the light-optical, immunocytochemistry and electron microscopy studies.Results. At conducting «hyamatrix» application conjunctiva edema and injection, corneal edema were reversed faster that reduced neovascularization risk in the outcome of corneal alkaline burn. Morphological studies at different periods of the experiment made it possible to determine the sequence of processes from the moment of cornea chemical burns application until the completion of its restoration, which were different in the experimental and control groups. Symptoms of toxic effect of the damaged cells decay products were determined morphologically. Endothelial cells remained intact. The use of «hyamatrix» application allowed reducing the recovery time with the improvement of cornea reparative processes.Conclusion. 1. Application of bioplastic material to the cornea in the early stages of cornea injuries treatment (alkaline and acid burn) reduces the severity of edema and hyperemia, and as a result reduces the exudative phase of inflammation. 2. As a result of clinical and functional analysis of course peculiarities of alkaline corneal damage found that «Hyamatrix» application accelerates corneal defect closure in 1.5 times faster as compared with the control group. 3. The use of biological material in the outcome of acid burn by reducing the exudative phase of inflammation accelerates the resorption of corneal opacity compared with the eye gel Solcoseryl application.
