IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE

Abstract

The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency of flexible oligonucleotide primers for 16S rDNA gene of gram-negative bacteria and also possibility of differentiation of these bacteria by use of the restriction endonuclease sets were shown.

Keywords

• PCR-RFLP
• 16s rRNA
• gram-negative (G-) bacteria