Antibacterial Activity of Biodegradable Films Incorporated with Biologically-Synthesized Silver Nanoparticles and the Evaluation of Their Migration to Chicken Meat
Meiriele da S. das Neves,
Sara Scandorieiro,
Giovana N. Pereira,
Jhonatan M. Ribeiro,
Amedea B. Seabra,
Adriana P. Dias,
Fabio Yamashita,
Claudia B. dos R. Martinez,
Renata K. T. Kobayashi,
Gerson Nakazato
Affiliations
Meiriele da S. das Neves
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Londrina State University, Londrina 86057-970, PR, Brazil
Sara Scandorieiro
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Londrina State University, Londrina 86057-970, PR, Brazil
Giovana N. Pereira
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Londrina State University, Londrina 86057-970, PR, Brazil
Jhonatan M. Ribeiro
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Londrina State University, Londrina 86057-970, PR, Brazil
Amedea B. Seabra
Center of Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, SP, Brazil
Adriana P. Dias
Department of Food Science and Technology, Londrina State University, Londrina 86057-970, PR, Brazil
Fabio Yamashita
Department of Food Science and Technology, Londrina State University, Londrina 86057-970, PR, Brazil
Claudia B. dos R. Martinez
Laboratory of Animal Ecophysiology, Department of Physiological Science, Londrina State University, Londrina 86057-970, PR, Brazil
Renata K. T. Kobayashi
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Londrina State University, Londrina 86057-970, PR, Brazil
Gerson Nakazato
Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Londrina State University, Londrina 86057-970, PR, Brazil
The food industry has been exploring the association of polymers with nanoparticles in packaging production, and active products are essential to increase the shelf life of food and avoid contamination. Our study developed starch-poly (adipate co-terephthalate butyl) films with silver nanoparticles produced with Fusarium oxysporum components (bio-AgNPs), intending to control foodborne pathogens. The bio-AgNPs showed activity against different Salmonella serotypes, including multidrug-resistant Salmonella Saint Paul and Salmonella Enteritidis, with minimum bactericidal concentrations ranging from 4.24 to 16.98 µg/mL. Biodegradable films with bio-AgNPs inhibited the growth of up to 106Salmonella isolates. Silver migration from the films to chicken was analyzed using electrothermal atomic absorption spectrophotometry, and the results showed migration values (12.94 mg/kg and 3.79 mg/kg) above the limits allowed by the European Food Safety Authority (EFSA) (0.05 mg/kg). Thus, it is necessary to improve the technique to avoid the migration of silver to chicken meat, since these concentrations can be harmful.
