Armaghane Danesh Bimonthly Journal (Jun 2018)
The Effect of Green Tea Hydroalcohalic Extract of Camellia Sinensis on AQP1 Gene Expression in Proximal Tubuls of Gentamicin Treated Rat kidney
Abstract
Background and Aim: Gentamicin is an antibiotic belong to a grouped of aminoglycoside used to treat a variety of bacterial infections particularly gram-negative ones. However, the nephrotoxicity and oxidative damage caused by gentamicin limit its long-term clinical use. Green tea contains some phenol compounds called catechins which have antioxidant and anti-inflammatory effects. AQP1 is proteins that make a water channel in renal tubules that transported water during the appical and baso-lateral of the renal tubules. The aim of this study was to evaluate the effect of green tea extract on expression of AQP1 gene in Gentamicin-induced renal toxicity in male Wistar rats. Methods: In this experimental study, 30 male Wistar rats aged 4-6 weeks (Weighing 160-200 grams) were divided into 5 groups of six: control group (no treatment), sham group (intraperitoneal injection of physiologic serum), group Experimental group 1 (intraperitoneal injection of 20 mg / kg gentamicin), experimental group 2 (intraperitoneal injection of green tea with a dose of 200 mg / kg), experimental group 3 (intraperitoneal injection of gentamicin and 200 mg / kg of green tea). All trated was performed intraperitoneally for 10 days. Blood biochemical changes included creatinine and urea levels based on Bertolt , Chlorometric and Gaffa method were determiend. At the end of exprement the renal changes were evaluated by means of histochemistry and immunohistochemistry. Data were analyzed by one-way ANOVA. Results: The mean serum level of urea and creatinine in group 1 was significantly increased in comparison with the control group (P<0.05) and the mean serum level of urea and creatinine in the third group was in comparison with the control group was significantly increased (P<0.05). Renal tissue necrosis and inflammation, accumulation of eosinophilic hyaline substances in some renal tubules, infiltration of inflammatory menonocellular cells and reduction of expression of AQP1 gene in the proximal tubule caused by gentamicin with a dosage of 200 mg / kg of green tea extract were not improved. Conclusion: From the findings of this study, it can be concluded that green tea extract in this dose and duration can not have a protective effect against the renal toxicity of gentamicin
