Preliminary Study Regard Histopathological Tissue Changes Associated to EDTA Experimental Exposure in Prussian Carp (Carassius Gibelio)

Abstract

The aim of the current study was to describe possible histopathological lesions induced by EDTA using as chelating agent in prussian carp (Carassius Gibelio) species. 80 healthy prussian carp specimens were collected from a local pond and acclimated for two weeks in laboratory conditions. Acclimated fish were housed in a 60 l capacity glass aquariums (20 fishes/aquarium) and divided into four groups: control group was maintained in EDTA-free freshwater, EDTA1 group receiving 0.05 g EDTA/l in water, EDTA2 group receiving 0.1g EDTA/l in water and EDTA3 group receiving 0.15 g EDTA/l in water. After a 21 days exposure to doses of EDTA above mentioned, some fish tissues were sampled and processed for microscopical examination. Compared to the control specimens, severe structural and functional alterations were identified in gills, liver and kidneys of fish exposed, including totally disorganized aspect of gill lamella with detached and dystrophic epithelium, intense fibrosis in the walls of arterioles, venules and gallbladder channels, denudation and necrosis processes of renal corpuscles respectively.

Keywords

• edta
• freshwater fish
• histopathological changes