Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training

Nasrin Ramezani; Behnaz Vanaky; Nader Shakeri; Zahra Soltanian; Fatemeh Fakhari Rad; Zahra Shams

Medical Laboratory Journal (Jan 2019)

Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training

Nasrin Ramezani,
Behnaz Vanaky,
Nader Shakeri,
Zahra Soltanian,
Fatemeh Fakhari Rad,
Zahra Shams

Affiliations

Nasrin Ramezani: Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Tehran University, Tehran, Iran
Behnaz Vanaky: Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
Nader Shakeri: Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
Zahra Soltanian: Department of Sport Physiology, Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
Fatemeh Fakhari Rad: Department of Sport Physiology, Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
Zahra Shams: Department of Sport Physiology, Faculty of Physical Education and Sports science, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Journal volume & issue: Vol. 13, no. 1
pp. 15 – 20

Abstract

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ABSTRACT Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity interval training on the expression of Bcl-2 and Bax as important apoptosis factors in diabetic rats. Methods: After inducing diabetes in 20 male Wistar rats (weighing 250 ± 1.15 g), the rats were randomly divided into a control group and a training group. The training group performed high-intensity interval training five days a week for four weeks, and the control group did not perform any training. After the intervention, RNA was extracted and TCF mRNA was subjected to real time RT-PCR for measuring Bax and Bcl-2 expression in the heart tissue of diabetic rats. Data were analyzed by IBM SPSS Statistics V22 using independent t-test. P-values less than 0.05 were considered as statistically significant. Results: The expression of Bcl-2 increased significantly and the expression of Bax decreased significantly after the four-week training intervention. Conclusion: The high-intensity interval training can have beneficial effects on the expression of apoptotic genes in rats with type 2 diabetes. Keywords: Bax, Bcl-2, High intensity interval training, Heart, Type 2 diabetes.

Published in Medical Laboratory Journal

ISSN: 2538-4449 (Online)
Publisher: Golestan University of Medical Sciences
Country of publisher: Iran, Islamic Republic of
LCC subjects: Medicine
Website: http://mlj.goums.ac.ir/index.php

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