This 6-page publication details the cultivation of oyster mushrooms from mother culture isolation to spawn preparation. This protocol can be used by both homeowners and commercial cultivators. Written by Chih-Ming Hsu, Khalid Hameed, Van T. Cotter, and Hui-Ling Liao and published by the UF/IFAS Department of Soil and Water Sciences, January 2018. http://edis.ifas.ufl.edu/ss663