Communications Biology (Jan 2023)

Split luciferase-based assay to detect botulinum neurotoxins using hiPSC-derived motor neurons

  • Laurent Cotter,
  • Feifan Yu,
  • Sylvain Roqueviere,
  • Juliette Duchesne de Lamotte,
  • Johannes Krupp,
  • Min Dong,
  • Camille Nicoleau

DOI
https://doi.org/10.1038/s42003-023-04495-w
Journal volume & issue
Vol. 6, no. 1
pp. 1 – 9

Abstract

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A cell-based assay for detecting SNAP25-cleaving Botulinum neurotoxins (BoNTs) is developed by combining hiPSC-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase.