In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouse

Abstract

Summary: Exosomes that contain various signaling molecules, such as proteins, nucleotides, metabolites, and lipids, are important for intercellular communication. Dendritic cells (DC) are central regulators of anti-tumor immunity but can be suppressed by tumor-derived exosomes (TDEs) in the tumor microenvironment. Here, we describe a step-by-step protocol for TDE isolation and evaluation of TDEs on DCs both in vitro and ex vivo with high repeatability. This approach is useful for the interrogating TDE-DC interactions and identification of novel immune regulators.For complete details on the use and execution of this protocol, please refer to Yin et al. (2020).

Keywords

• Cancer
• Cell-based assays
• Flow cytometry/mass cytometry
• Immunology