Hsa‐miR‐335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine‐1
Yang Wang,
Jing Zhang,
Li‐Ye Zhong,
Shang‐Jia Huang,
Nan‐Nan Yu,
Lan Ouyang,
Yu‐Long Niu,
Jun‐Xiong Chen,
Chun‐Hua Lu,
Qing‐Yu He
Affiliations
Yang Wang
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Jing Zhang
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Li‐Ye Zhong
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Shang‐Jia Huang
Gastrointestinal Surgery The First People's Hospital of FoShan Foshan China
Nan‐Nan Yu
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Lan Ouyang
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Yu‐Long Niu
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Jun‐Xiong Chen
Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital Sun Yat‐sen University Guangzhou Guangdong China
Chun‐Hua Lu
Research Laboratory of Zhuang & Yao Medicine Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine Nanning Guangxi China
Qing‐Yu He
MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology Jinan University Guangzhou China
Abstract Lung adenocarcinoma (LAC) is one of the most common pulmonary adenocarcinomas with a high peak of mortality, and metastasis is the main culprit of LAC deaths. microRNAs play important role in cancer metastasis, and thus are regarded as potential diagnostic and prognostic markers for human cancers. However, many miRNAs exhibit dual roles in diverse cellular contexts. Here, we revealed that hsa‐miR‐335, a previously reported tumor suppressor, exhibited an oncogenic role in LAC. Overexpression of miR‐335 enhanced the abilities of A549 and H1299 cells to invade and migrate by regulating epithelial‐mesenchymal transition, while inhibition of miR‐335 exhibited an opposite effect in vitro and in vivo. Mechanically, miR‐335 inhibited the expression of Copine‐1 (CPNE1), an NF‐κB suppressor, through interacting with its mRNA 3′UTR, while mutating the binding sites abolished this inhibitory effect. This finding not only highlights the suppressive effect of CPNE1 on cell motility, but also provides new insight into miR‐335 in promoting LAC metastasis.
