In vitro Regeneration Studies of Vuralia turcica Using Unpollinated Ovaries

Abstract

Vuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress. However, the maintenance of this species is currently challenging because of climatic changes in the plant’s habitat, intensive agricultural purposes, grazing, and the like. Gynogenesis is a promising method for micropropagation and a useful technic for genetic engineering. The objective of this study was to analyze the gynogenesis of V. turcica. The experiments described here were implemented during the flowering time of V. turcica (spring) using unpollinated ovaries. Modified MS and B5 induction media and different plant growth regulators (BAP, GA3, 2,4-D, and KIN) were compared. The comparison of both media (MS and B5) showed that MS medium supplemented with 2,4-D and KIN was the best medium for in vitro unfertilized ovary culture and gave the best result of regenerated plants per 30 ovaries, and 92% callus was obtained. Only the control groups showed very low scores or no plants. From plant regeneration and subcultures, a total of 60 plants were produced, all green. These results are of interest to the in vitro conservation of endangered plant species like V. turcica.

Keywords

• b5
• legumes
• ms
• pure lines
• ovary culture