Protective Effects on 60Co-γ Radiation Damage of Pine Cone Polyphenols from Pinus koraiensis-Loaded Chitosan Microspheres In Vivo
Sujuan Shao,
Juanjuan Yi,
Joe M. Regenstein,
Cuilin Cheng,
Hua Zhang,
Haitian Zhao,
Zhenyu Wang
Affiliations
Sujuan Shao
Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Nangang District, Harbin 150090, China
Juanjuan Yi
School of Life Sciences, Zhengzhou University, Zhengzhou 450000, Henan, China
Joe M. Regenstein
Department of Food Science, Cornell University, Ithaca, NY 14853-7201, USA
Cuilin Cheng
Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Nangang District, Harbin 150090, China
Hua Zhang
Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Nangang District, Harbin 150090, China
Haitian Zhao
Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Nangang District, Harbin 150090, China
Zhenyu Wang
Department of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Nangang District, Harbin 150090, China
A novel chitosan microsphere for encapsulating pine cone polyphenols (PP) from P. koraiensis was successfully prepared using an emulsion crosslinking technique. The characteristics of pine polyphenol-loaded microspheres (PPM) were determined using scanning electron microscopy (SEM) and a laser particle size detector. It was found that PPMs were spherical in shape with uniform particle size distribution patterns. The drug content and encapsulation rate of the microspheres were 7.47% and 73.6%, respectively, at a Ch/GA mass ratio of 0.7. The animal experiments showed that PPM had a stronger radiation protective effect than PP. PPM significantly increased the immune organ indices, the quantity of marrow DNA, the superoxide dismutase (SOD) activity, the splenocyte proliferation index, and the phagocytosis activity of monocytes. PPM also decreased the numbers of micronuclei in bone marrow cells and malondialdehyde (MDA) levels in plasma in mice exposed to 60Co γ-irradiation. In addition, gender differences in biological responses to exposure to radiation were observed.
